Purification and characterization of phytase from <i>Bacillus subtilis</i> P6: Evaluation for probiotic potential for possible application in animal feed

The antinutritional property of phytate is the main problem associated with food animals, but phytase-producing probiotic strain could be best alternative for resolving this problem. In present study, phytase from Bacillus subtilis P6 was purified to homogeneity, which showed a relative molecular mass 40 kDa on native and SDS-PAGE. purification fold 86.99, specific activity 104.39 U/mg protein. enzyme optimum at 40°C pH 6.0. Semi-metal ions, such as K+ Ca2+, slightly enhance while Cu2+, Co2+, Zn2+ prominently inhibited activity. Purified highest substrate specificity toward its natural did not hydrolyze other substrates having phosphate bond. exhibited strong proteolytic tolerance against trypsin pepsin. Further, we evaluated potential B. noted that it excellent resistance high acidic bile concentrations. Subsequently, revealed exceptional antimicrobial activity, good hydrophobicity, autoaggregation ability, nonhemolytic, nonlecithinase Additionally, displayed susceptibility major antibiotics. Owing biochemical properties notable characteristics, may offer possibility being promising candidate application in animal feed supplements. However, vivo use secreting needs studied animals intended benefits